Keepers are responsible for the wellbeing of a group of animals in their care. The type of animal and range of duties depends on what section of Perth Zoo they work in.

As an example, a Keeper working with Perth Zoo's Australian Fauna section would have the following duties:

• Provides general care and husbandry and monitors the health of Australian Mammals, Reptiles and Birds and reports any problems to the supervisor.
• Prepares appropriate food for a wide range of species in accordance with established diets, feeding schedules and procedures.
• Ensures that animal exhibits are clean and safe and assists in refurbishing and landscaping.
• Captures, restrains and handles a wide range of species as required.
• Assists Veterinary Department staff with the administration of medication and testing of animals.
• Assists with research projects and data collection.
• Provides an education and information service to the public on the Zoo's activities.
• Maintains exhibit and animal records and provide reports.
• Participates in effective human resource management, Equal Employment Opportunity and Occupational Health Welfare and Safety guidelines and policies.